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KMID : 0545120170270101827
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Journal of Microbiology and Biotechnology
2017 Volume.27 No. 10 p.1827 ~ p.1836
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Bee Venom Decreases LPS-Induced Inflammatory Responses in Bovine Mammary Epithelial Cells
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Jeong Chang-Hee
Cheng Wei Nee
Bae Hyo-Jin
Lee Kyung-Woo
Han Sang-Mi
Petriello Michael C.
Lee Hong-Gu
Seo Han-Geuk
Han Sung-Gu
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Abstract
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The world dairy industry has long been challenged by bovine mastitis, an inflammatory disease, which causes economic loss due to decreased milk production and quality. Attempts have been made to prevent or treat this disease with multiple approaches, primarily through increased abuse of antibiotics, but effective natural solutions remain elusive. Bee venom (BV) contains a variety of peptides (e.g., melittin) and shows multiple bioactivities, including prevention of inflammation. Thus, in the current study, it was hypothesized that BV can reduce inflammation in bovine mammary epithelial cells (MAC-T). To examine the hypothesis, cells were treated with LPS (1 μg/ml) to induce an inflammatory response and the antiinflammatory effects of BV (2.5 and 5 μg/ml) were investigated. The cellular mechanisms of BV against LPS-induced inflammation were also investigated. Results showed that BV can attenuate expression of an inflammatory protein, COX2, and pro-inflammatory cytokines such as IL-6 and TNF-α. Activation of NF-κB, an inflammatory transcription factor, was significantly downregulated by BV in cells treated with LPS, through dephosphorylation of ERK1/2. Moreover, pretreatment of cells with BV attenuated LPS-induced production of intracellular reactive oxygen species (e.g., superoxide anion). These results support our hypothesis that BV can decrease LPS-induced inflammatory responses in bovine mammary epithelial cells through inhibition of oxidative stress, NF-κB, ERK1/2, and COX-2 signaling.
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KEYWORD
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Bee venom, inflammation, mammary epithelial cells, bovine mastitis, lipopolysaccharide
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